Author: Pipkin, K.M.; Hagey, J.V.; Rayburn, M.C.; Chigerwe, M.
Title: A Randomized Clinical Trial Evaluating Metabolism of Colostral and Plasma Derived Immunoglobulin G in Jersey Bull Calves Document date: 2015_4_9
ID: 7nmaf6u0_3
Snippet: Blood and fecal samples from each calf were collected by jugular venipuncture and digitally, respectively, at 0 h (before procedures), 6 h, 12 h, 24 h, 48 h, 5 d, and 7 d of age. Serum was harvested from the blood samples after centrifugation at 2,880 9 g for 5 min at 4°C. Serum total protein concentration was determined by a hand-held refractometer. c Serum samples were then stored at À20°C until IgG determination. Fecal samples were immediat.....
Document: Blood and fecal samples from each calf were collected by jugular venipuncture and digitally, respectively, at 0 h (before procedures), 6 h, 12 h, 24 h, 48 h, 5 d, and 7 d of age. Serum was harvested from the blood samples after centrifugation at 2,880 9 g for 5 min at 4°C. Serum total protein concentration was determined by a hand-held refractometer. c Serum samples were then stored at À20°C until IgG determination. Fecal samples were immediately frozen at À20°C until IgG determination. Serum and fecal IgG determinations were performed by single radial immunodiffusion (SRID). All calves were enrolled within a 2-wk period and the study was performed from June 2014 to July 2014.
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