Author: Hoang, Minh; Wu, Hung-Yi; Lien, Ying-Xiu; Chiou, Ming-Tang; Lin, Chao-Nan
Title: A SimpleProbe(®) real-time PCR assay for differentiating the canine parvovirus type 2 genotype Document date: 2018_8_31
ID: 407bgxzx_10
Snippet: The detection limits of the assay were evaluated using 10-fold dilution replicates of plasmids 2a, 2b and 2c, corresponding to an input equivalent to 10 2 -10 6 DNA copies/μL. The 10-fold-diluted plasmid tests showed that the SimpleProbe ® assay detected approximately 10 4 DNA copies/μL (Table 3) . Moreover, DNA extracted from canine circovirus, canine coronavirus, canine distemper virus, calicivirus, and rabies virus did not interfere with th.....
Document: The detection limits of the assay were evaluated using 10-fold dilution replicates of plasmids 2a, 2b and 2c, corresponding to an input equivalent to 10 2 -10 6 DNA copies/μL. The 10-fold-diluted plasmid tests showed that the SimpleProbe ® assay detected approximately 10 4 DNA copies/μL (Table 3) . Moreover, DNA extracted from canine circovirus, canine coronavirus, canine distemper virus, calicivirus, and rabies virus did not interfere with the assay ( Figure S1 ).
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