Author: Hoang, Minh; Wu, Hung-Yi; Lien, Ying-Xiu; Chiou, Ming-Tang; Lin, Chao-Nan
Title: A SimpleProbe(®) real-time PCR assay for differentiating the canine parvovirus type 2 genotype Document date: 2018_8_31
ID: 407bgxzx_8
Snippet: The SimpleProbe ® (TIB MOLBIOLGmbH, Berlin, Germany) was designed based on specific features of the CPV-2b variant. The primers and probe sequences are listed in Table 1 temperature from 40°C to 75°C. The sensitivity and performance of the SimpleProbe ® assay were evaluated using 10 replications of 10-fold-diluted control plasmid from 10 2 -10 6 , from which the realtime PCR detection limit was determined. In addition, the specificity of the .....
Document: The SimpleProbe ® (TIB MOLBIOLGmbH, Berlin, Germany) was designed based on specific features of the CPV-2b variant. The primers and probe sequences are listed in Table 1 temperature from 40°C to 75°C. The sensitivity and performance of the SimpleProbe ® assay were evaluated using 10 replications of 10-fold-diluted control plasmid from 10 2 -10 6 , from which the realtime PCR detection limit was determined. In addition, the specificity of the SimplePrboe ® assay was also assessed by testing nucleic Figure 1 ).
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