Author: SAKAI, Kosei; YONEZAWA, Tomohiro; YAMAWAKI, Hideyuki; OYAMADA, Toshifumi
Title: Alteration of somatostatin receptor 2 expression in canine mammary gland tumor Document date: 2015_5_18
ID: 765ufb5i_4
Snippet: For Western blotting, 12 canine mammary gland tissues (4 normal, 4 simple adenomas, one complex adenoma and 3 simple carcinomas), harvested and flash frozen in liquid nitrogen, were obtained either from experimental animals or from patients undergoing surgery for mammary gland tumors at the Kitasato University Veterinary Teaching Hospital. We performed Western blotting analysis according to a previously reported protocol [12] . Twelve micrograms .....
Document: For Western blotting, 12 canine mammary gland tissues (4 normal, 4 simple adenomas, one complex adenoma and 3 simple carcinomas), harvested and flash frozen in liquid nitrogen, were obtained either from experimental animals or from patients undergoing surgery for mammary gland tumors at the Kitasato University Veterinary Teaching Hospital. We performed Western blotting analysis according to a previously reported protocol [12] . Twelve micrograms of protein were loaded in each well. The human SSTR2 antibody used in immunohistochemistry analysis (1:300 dilution) was also used in Western blotting. Samples were visualized using horseradish peroxidase-conjugated secondary antibodies and the EZ-ECL system (Biological Industries, Kibbutz Beit Hesmek, Israel). The expression level of SSTR2 in each sample was normalized to total actin (1:500 dilution, MAB1501, Sigma-Aldrich, St. Louis, MO, U.S.A.) expression on the same membrane as an internal control.
Search related documents:
Co phrase search for related documents- blotting analysis and immunohistochemistry analysis: 1, 2
- blotting analysis and secondary antibody: 1, 2
- blotting analysis and western blotting: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73
- blotting analysis and western blotting analysis: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53, 54, 55, 56, 57, 58, 59, 60, 61, 62, 63, 64, 65, 66, 67, 68, 69, 70, 71, 72, 73
- blotting analysis and western blotting analysis perform: 1
- complex adenoma and simple adenoma: 1, 2
- complex adenoma and simple carcinoma: 1
- experimental animal and expression level: 1, 2, 3, 4
- experimental animal and liquid nitrogen: 1
- experimental animal and western blotting: 1, 2
- expression level and gland tumor: 1
- expression level and immunohistochemistry analysis: 1, 2, 3, 4
- expression level and internal control: 1, 2, 3
- expression level and mammary gland tumor: 1
- expression level and secondary antibody: 1, 2, 3
- expression level and western blotting: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48
- expression level and western blotting analysis: 1, 2, 3, 4, 5, 6, 7
- gland tissue and mammary gland tissue: 1, 2, 3, 4, 5, 6, 7, 8
- gland tissue and secondary antibody: 1
Co phrase search for related documents, hyperlinks ordered by date