Author: Joshua A. Hayward; Mary Tachedjian; Adam Johnson; Tamsin B. Gordon; Jie Cui; Glenn A. Marsh; Michelle L. Baker; Lin-Fa Wang; Gilda Tachedjian
Title: Bats Possess Unique Variants of the Antiviral Restriction Factor Tetherin Document date: 2020_4_9
ID: eksi9yia_60
Snippet: Amplicons from PCR reactions were analysed by agarose gel electrophoresis (57) . Individual DNA bands were physically excised from the gel. DNA was purified from the gel fragments The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.08.031203 doi: bioRxiv preprint standard heat-shock method (57) . Plasmids were purified from these cultures using a Wizard Plus SV Miniprep DNA Purification ki.....
Document: Amplicons from PCR reactions were analysed by agarose gel electrophoresis (57) . Individual DNA bands were physically excised from the gel. DNA was purified from the gel fragments The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.08.031203 doi: bioRxiv preprint standard heat-shock method (57) . Plasmids were purified from these cultures using a Wizard Plus SV Miniprep DNA Purification kit (Promega). All inserted sequences were confirmed by Sanger sequencing using M13 forward and reverse primers (M13F and M13R; Table 3B ).
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