2015 ACVIM Forum Research Abstract Program

At all time points, there were statistically significant differences in lactate concentrations within the anaerobic chilled samples (gold standard group). The aerobic chilled samples did not have statistically significant change in lactate when compared to anaerobic chilled at the same time points. The anaerobic RT samples had statistically significant increases at all time points after 15-25 minute. The aerobic RT samples had statistically significant changes in lactate at all time points. Results support analyzing lactate samples immediately. Findings indicate that time, temperature and storage conditions all cause significant changes in lactate. If lactate analysis is delayed, anaerobic chilled samples should be analyzed within 70 minutes, and aerobic chilled samples should be analyzed within 25 minutes. Aerobic RT & anaerobic RT sample storage should be avoided. Clinicians should be aware that pre-analytical factors including storage time and sampling handling may cause changes in lactate values. The aim of this study was to determine if cats with urinary obstruction have an inflammatory acute phase response. For that, the concentrations of haptoglobin (HP) and serum amyloid A (SAA) were determined in cats with clinically urinary tract obstruction at the time of diagnosis. Thirty-four cats were included in the study. The animals were between 6 months to 15 years old. Cats were assigned to groups according to clinical signs, hemogram and urinalysis. 16 cats showed clinical signs of urinary tract obstruction and 18 cats were clinically healthy control cats. The HP serum concentrations were measured via haemoglobin binding assay modified for automation on an ABX Pentra Analyser (Horiba Medical, Montpellier, France). SAA concentrations were determined using LAT Eiken Chemical Co. (Japan) modified for automation on ABX Pentra Analyser. The HP concentrations in the control group ranged from 2.02 to 4.12 g/L (median 3.08 g/L) and SAA concentrations in this group were all <5 mg/L. In the clinically obstructed cats the HP concentrations ranged from 2.5 to 6.04 g/L (median 4.04 g/L), SAA concentrations ranged from <5 to 228.5 mg/L (median <5 mg/L), white blood cells (WBC) ranged from 7100 to 24,200/ lL (median 12,195/lL) and neutrophils ranged from 5760 to 18,030/lL (median 11,320/lL). All these parameters were significantly higher (P < 0.05) when compared to the clinically healthy cats. The increases seen in HP and SAA values were accompanied by increases in WBC and neutrophils in the clinically obstructed cats demonstrating an acute phase response in cats with urinary tract obstruction. However, there was no correlation between HP and WBC indicating that these analytes respond with differing dynamics to urinary obstruction and that measurement of acute phase protein could add value to the diagnostic regime for this condition in cats. Serum amyloid A (SAA) is a major acute phase protein in many species. SAA has been reported to be a sensitive biomarker of inflammation in dogs, cats, and horses. The high level of identity between SAA from different species allows measurement of canine, feline and equine SAA using the same immunoassay. The aim of this study was to select antibodies with cross-reactivity for canine, feline and equine SAA from the panel of murine monoclonal antibodies (mAbs) developed against either human or canine SAA. The study also included development of recombinant species-specific calibrators for SAA immunoassays.