2016 ACVIM Forum Research Abstract Program

Forty-four dogs with FRD and 20 dogs with SRD were included in the study. Granulocyte Mosaic Biochip slides (EURO-IMMUN AG) containing human granulocytes fixed in ethanol, human granulocytes fixed in formalin, purified myeloperoxidase protein (MPO), purified proteinase-3 protein (PR-3), and HEp-2 cells were used for the assay. These biochips allow detection of pANCA, cytoplasmic antineutrophil antibodies (cANCA), as well as anti-nuclear antibodies (ANA) on the same slide. The assay was performed according to manufacturer's instructions. Briefly, biochips were incubated with 30ul of 1:10 and 1:100 diluted serum samples for 30 Min, then washed and incubated with secondary anti-dog IgG FITC-labeled antibody for 30 Min. After washing, the slides were embedded in medium, covered and evaluated under fluorescence light at 20-409 magnification by two independent observers.