Title: 2016 ACVIM Forum Research Abstract Program Document date: 2016_5_31
ID: 2y1y8jpx_372
Snippet: Quantitative real-time PCR (qRT-PCR) was used to assess BAFF mRNA expression. Total RNA was isolated from 6 dogs with untreated pITP, 5 healthy dogs, and 15 dogs with secondary thrombocytopenia. pITP was defined as dogs that had platelet counts <20,000 cells/mL, were PCR and serology negative for Babesia, Ehrlichia, and Anaplasma, had no evidence of neoplasia on abdominal and thoracic imaging, and no history of drug administration (antibiotics, c.....
Document: Quantitative real-time PCR (qRT-PCR) was used to assess BAFF mRNA expression. Total RNA was isolated from 6 dogs with untreated pITP, 5 healthy dogs, and 15 dogs with secondary thrombocytopenia. pITP was defined as dogs that had platelet counts <20,000 cells/mL, were PCR and serology negative for Babesia, Ehrlichia, and Anaplasma, had no evidence of neoplasia on abdominal and thoracic imaging, and no history of drug administration (antibiotics, corticosteroids, vaccines, or chemotherapeutics). Secondary thrombocytopenic control dogs had platelet counts below the reference interval due to diagnoses other than pITP, and healthy dogs presented for wellness exams and had normal platelet counts. qRT-PCR was performed using intron-spanning primers to amplify a 129 base pair region of the mRNA. b-actin was used as a reference gene.
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