2016 ACVIM Forum Research Abstract Program

Hye-Bin Wang 1 , Hyeri Shin 1 , Jin-Young Chung 2 , Kun-Ho Song 1 , Kyoung-Won Seo 1 . 1 Chungnam National University, Daejeon, Korea, 2 Kangwon National University, Chuncheon, Korea Thromboelastography (TEG) is rapid and non-invasive diagnostic instrument that has been used in veterinary medicine for evaluation of haemostatic function in whole blood (WB). Recently, rapid thromboelastography (r-TEG) has been recognized as an overall evaluation of coagulation abnormalities in human medicine and become available faster than conventional coagulation tests (CCTs). Although r-TEG is becoming more generally used in human medicine, there are no studies describing the use of r-TEG in veterinary medicine. The objectives of this study were to 1) validation of r-TEG values with native and citrated WB samples from healthy dogs, and to compare stability and repeatability between two types of samples, and 2) examine whether the use of r-TEG could accelerate the coagulation pathway and decrease in taken time in comparison to kaolin activated TEG. Blood was collected from 16 clinically healthy Beagle dogs. TEG analysis was performed on kaolin-activated, citrated r-TEG, and native r-TEG blood samples. TEG activated clotting time (ACT), kappa value (k), alpha angle (a), and maximum amplitude (MA) were recorded. Coefficients of variation (CV) of native r-TEG and citrate r-TEG for TEG ACT, K, a angle, and MA were 13.4/ 18.8%, 11.1/16.6%, 4.2/5.1% and 10.0/10/0%, respectively. The mean time for the citrated and native r-TEG were 1313.9 AE 250.9/ 1351.3 AE 264.6 seconds (mean AE SD) respectively, in comparison with 1779.9 AE 197.0 seconds for kaolin activated TEG.