Title: 2016 ACVIM Forum Research Abstract Program Document date: 2016_5_31
ID: 2y1y8jpx_439
Snippet: Whole blood was collected from 260 pit bull-type dogs rescued during a federal dogfighting investigation. Real-time PCR was performed for Babesia species, and positive samples underwent a second round of species-specific PCR in a commercial diagnostic laboratory (IDEXX Laboratories, Inc.). Residual whole blood samples were frozen and stored at -80 C pending subsequent analysis. POCKIT Ã’ iiPCR was performed twice on each sample, once with a kit s.....
Document: Whole blood was collected from 260 pit bull-type dogs rescued during a federal dogfighting investigation. Real-time PCR was performed for Babesia species, and positive samples underwent a second round of species-specific PCR in a commercial diagnostic laboratory (IDEXX Laboratories, Inc.). Residual whole blood samples were frozen and stored at -80 C pending subsequent analysis. POCKIT Ã’ iiPCR was performed twice on each sample, once with a kit specific for B. gibsoni and once with a kit that detects both B. gibsoni and Babesia canis (Babesiosis). The sensitivity and specificity of each test were calculated using the commercial test as the gold standard. Positive (PPV) and negative (NPV) predictive values were calculated for theoretical 5%, 15%, and 30% prevalences. The study was approved by the UF IACUC.
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