Author: Yun, Sang-Im; Song, Byung-Hak; Kim, Jin-Kyoung; Lee, Young-Min
Title: Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses Document date: 2015_12_29
ID: 2se4d1yp_4
Snippet: Step 1, purification of viral RNA from the cell culture supernatant of JEV-infected BHK-21 cells (Figure 1A) ; Step 2, synthesis of four overlapping cDNA amplicons (F1 to F4) spanning the whole viral genome ( Figure 1B); Step 3, subcloning of each of the four contiguous cDNA fragments into a BAC vector, creating pBAC/F1 to pBAC/F4 ( Figure 1C) ;.....
Document: Step 1, purification of viral RNA from the cell culture supernatant of JEV-infected BHK-21 cells (Figure 1A) ; Step 2, synthesis of four overlapping cDNA amplicons (F1 to F4) spanning the whole viral genome ( Figure 1B); Step 3, subcloning of each of the four contiguous cDNA fragments into a BAC vector, creating pBAC/F1 to pBAC/F4 ( Figure 1C) ;
Search related documents:
Co phrase search for related documents- BAC vector and cell culture: 1
- BAC vector and viral genome: 1, 2
- cdna fragment and cell culture: 1, 2, 3, 4
- cdna fragment and viral genome: 1, 2
- cell culture and viral genome: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25
- cell culture and viral RNA purification: 1, 2
Co phrase search for related documents, hyperlinks ordered by date