Author: Yun, Sang-Im; Song, Byung-Hak; Kim, Jin-Kyoung; Lee, Young-Min
Title: Bacterial Artificial Chromosomes: A Functional Genomics Tool for the Study of Positive-strand RNA Viruses Document date: 2015_12_29
ID: 2se4d1yp_4
Snippet: Step 1, purification of viral RNA from the cell culture supernatant of JEV-infected BHK-21 cells (Figure 1A) ; Step 2, synthesis of four overlapping cDNA amplicons (F1 to F4) spanning the whole viral genome ( Figure 1B); Step 3, subcloning of each of the four contiguous cDNA fragments into a BAC vector, creating pBAC/F1 to pBAC/F4 ( Figure 1C) ;.....
Document: Step 1, purification of viral RNA from the cell culture supernatant of JEV-infected BHK-21 cells (Figure 1A) ; Step 2, synthesis of four overlapping cDNA amplicons (F1 to F4) spanning the whole viral genome ( Figure 1B); Step 3, subcloning of each of the four contiguous cDNA fragments into a BAC vector, creating pBAC/F1 to pBAC/F4 ( Figure 1C) ;
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