2016 ACVIM Forum Research Abstract Program

Genevieve Clark, Roberta Cahill, Brendon Thatcher, Peter F. Smith, Thomas P. O'Connor, Ramaswamy Chandrashekar, Melissa J. Beall, Jesse S. Buch. IDEXX Laboratories, Inc., Westbrook, Maine, USA Feline leukemia virus (FeLV) is a globally-distributed oncogenic retrovirus that can cause fatal disease in cats. Immunoassays that detect FeLV p27 antigen and are readily used to aid in the diagnosis of FeLV have been commercially-available for more than two decades. While single-sample rapid test formats are preferred for in-clinic use, the microtiter plate format enzyme-linked immunosorbent assay (ELISA) is the methodology most commonly employed in reference laboratory settings to facilitate batch testing. However, current commercially-available microtiter plate format ELISAs for FeLV are not well suited for this purpose because they rely on manual wash steps, drop-wise addition of reagents from reagent bottles, and visual interpretation of results. The purpose of this study was to validate a microtiter plate format ELISA designed specifically to maintain performance while enhancing throughput in a reference laboratory setting.