Selected article for: "dna extraction and single nucleotide polymorphism"

Title: 2015 ACVIM Forum Research Abstract Program
  • Document date: 2015_5_27
  • ID: 3pnuj5ru_132
    Snippet: In this study, a population of 100 Persian cats was assessed by: echocardiography, electrocardiography, laboratorial tests and blood pressure determination. The animals were classified according to the presence or not of HCM. Blood samples from experimental groups (20 older cats without HCM and 22 cats with HCM) were subjected to DNA extraction, genotyping by PCR and sequencing of cardiac alpha-actin gene (exon 5 of ACTC1) and myosin binding prot.....
    Document: In this study, a population of 100 Persian cats was assessed by: echocardiography, electrocardiography, laboratorial tests and blood pressure determination. The animals were classified according to the presence or not of HCM. Blood samples from experimental groups (20 older cats without HCM and 22 cats with HCM) were subjected to DNA extraction, genotyping by PCR and sequencing of cardiac alpha-actin gene (exon 5 of ACTC1) and myosin binding protein C gene (exon 27 of MYBPC3) with subsequent correlation with the presence of mutations and HCM. In the evaluated population, HCM was more prevalent in older and male cats; it occurred in 22 animals; and the asymmetric hypertrophy at basal region of septum was the most common. One single nucleotide polymorphism (SNP) at position 890 of exon 5 of the gene ACTC1 and three SNP in intron 5-6 of the same gene were identified. No polymorphism, addition or dele-tion was observed in other regions of the gene ACTC1 or MY-BPC3 gene.

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