2015 ACVIM Forum Research Abstract Program

ut is not found in G0. Metal-lothionein (MT) is a low molecular weight protein that increases significantly during oxidative stress to protect the cells against citotoxicity and DNA damage and these properties contribute to the anti-apoptotic function. MT as well as Ki-67 represents a poor prognostic factor in DLBCL. The goal of this study was investigate Ki-67 and MT expression in cats with alimentary lymphoma. Immunohistochemistry in paraffin samples was performed in 16 cats with alimentary lymphoma without previous treatment, using monoclonal antibodies: mouse anti-PAX-5 (PAX5 Clone 24; Invitrogen); polyclonal rabbit anti-CD3 (DAKO); mouse anti-Ki67 (clone MIB-1; DAKO); and mouse anti-metallothionein (clone E9; DAKO). The Ki-67 immunoreactivity was determined by count 1,000 cells in 40X fields, while metallothionein was analyzed by degree of labeling intensity between 0 (no expression) to 3 (strong expression). Six cats had B-cell (one small cell and 5 large cells) and 10 had T-cell (small cell) lymphoma. The mean of Ki-67 expression was 60.5% in cats with B-cell and 10.8% in T-cell lymphoma (p < 0.0001), and the median of survival time was 3 and 27 months, respectively. Only one cat expressed MT (grade 2) associated with low levels of Ki-67 (5%) and had a long survival time. In conclusion Ki-67 had relation with immunophenotype and survival time and MT was rarely express in alimentary lymphoma in cats. Surgical resection of the primary tumor is an effective treatment for mammary neoplasms in dogs. Nevertheless, the surgical stress response may provide conditions for persistence of residual minimal disease and the development of pre-existing micrometastases. Several mechanisms underlying these processes have been proposed, including postoperative suppression of cell-mediated immunity, the first-line defense mechanism against cancer. Existing data in human patients suggest that an increase in the level of interleukin 6 (IL-6) and interleukin 8 (IL-8), in combination with a decrease of T helper cell-induced cytokine production, interleukin 2 (IL-1) and interferon gama (IFN-c), could account for the profound suppression of natural killer cytotoxic activity in the perioperative period. Given the lack of related studies evaluating the immune response associated with mastectomy in dogs, the aim of this study was to evaluate the serum concentration of IL-2 and IL-6 in the perioperative period of unilateral total mastectomy (UTM) alone or in association with ovariosalpingohisterectomy (OSH) in bitches with mammary tumors.