Selected article for: "fecal microbiota and rrna gene"
Title: 2015 ACVIM Forum Research Abstract Program
  • Document date: 2015_5_27
    • ID: 3pnuj5ru_343
    Snippet: Healthy dogs were randomized to receive either the probiotic SIVOY (n = 10) or a placebo (n = 10). Fecal samples were collected at baseline (T0) and after 60 days (T60) of supplementation. DNA was extracted using the ZR Fecal DNA Kit TM (Zymo Research Corporation, Irvine, CA). The fecal microbiota was analyzed by Illumina sequencing of the 16S rRNA gene. Quanti-tative PCR was performed for Lactobacillus spp., Bifidobacterium spp., E. coli, C. per.....
    Document: Healthy dogs were randomized to receive either the probiotic SIVOY (n = 10) or a placebo (n = 10). Fecal samples were collected at baseline (T0) and after 60 days (T60) of supplementation. DNA was extracted using the ZR Fecal DNA Kit TM (Zymo Research Corporation, Irvine, CA). The fecal microbiota was analyzed by Illumina sequencing of the 16S rRNA gene. Quanti-tative PCR was performed for Lactobacillus spp., Bifidobacterium spp., E. coli, C. perfringens, and Streptococcus spp. Sequence percentages and qPCR data between time points were evaluated using a Wilcoxon rank-sum test. To determine differences in microbial communities between groups, the analysis of similarities (ANOSIM) function in QIIME (pipeline version 1.8) was used based on the unweighted UniFrac distance matrixes. Statistical significance was set at p < 0.05.

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