2015 ACVIM Forum Research Abstract Program

Dogs of any size may develop IMPA, usually in warm months. Owners often but not always recognized lameness. A good response to therapy was common, but remission was less likely with type II-IV IMPA. The purpose of the study was to determine the effects of various storage conditions on canine lactate concentrations. Prospectively 12 mL blood was collected form 30 healthy dogs. The blood was promptly divided into storage aliquots that were each used once. Storage conditions consisted of a gold standard (anaerobic chilled), and three comparator groups (aerobic chilled, anaerobic room temperature (RT), aerobic RT). Time points of analysis were T0, T15-25, T30-40, T60-70 and T120-130 minute in the gold standard group; and T15-25, T30-40, T60-70 and T120-130 minute in the comparator groups. Nonparametric statistics were used. To assess clinical significance all values were then compared to the gold standard by calculating absolute and percent differences versus T0. A mean change >20% from T0 values was considered to be clinically unacceptable.