2015 ACVIM Forum Research Abstract Program

The index dog was blood typed DEA 1 + and 4 + (Dal-), while the blood donor was DEA 4 + and Dal+. The transfusion was uneventful with no clinical and/or laboratory signs of hemolytic transfusion reaction observed. The PCV/TS remained stable over the time, except for an expected increased PCV 2 hours post-transfusion (55%), which returned to its initial value 24 hours later (38%). All Coombs' tests were negative. A weak positive anti-Dal agglutination reaction was first observed at day 4 (titer of 1:1). The agglutination titer progressed thereafter, reaching a maximum of 1:64 at week 8, followed by a gradual decrease one year post-transfusion to 1:8. The anti-Dal antibody appeared to be of the IgG class. Anti-Dal antibodies produced prior to day 28 were no longer detected following a 6-months freezing period. However, freezing did not cause any decrease in agglutination titer of anti-Dal antibodies collected on day 28 and after, i.e. the titer level was thereafter useful for Dal-typing purposes. All 65 dogs blood typed for Dal had concordant results using both polyclonal anti-Dal antibodies (25 Dal-and 40 Dal+).