2015 ACVIM Forum Research Abstract Program

Sixteen cats with cytologically diagnosed FHL were divided into two groups: Group 1: 9 cats with no definitively diagnosed underlying disease (4À11 years; 6 FS; 3 MC) and Group 2: 7 cats with a definitively diagnosed underlying disease ( . Serum folate and cobalamin concentrations were measured using an automated chemiluminescence assay. Serum MMA and HCY concentrations were measured using in-house GC/MS assays. The prevalence of hypocobalaminemia (reference interval: 290À1,500 ng/L) and hypofolatemia (reference interval: 9.7À21.6 lg/L) were compared among the three groups using v 2 tests. Serum MMA and HCY concentrations were compared amongst the three groups using ANOVA or Kruskal-Wallis tests, as appropriate. Statistical significance was set as P < 0.05. The prevalence of hypocobalaminemia was as follows: 6%, 13%, and 0% for Groups 1, 2, and 3, respectively (P = 0.06). The prevalence of hypofolatemia was as follows: 31%, 25%, and 10% for Groups 1, 2 and 3, respectively (P = 0.12). Serum cobalamin concentrations (mean AESD) were lower in Group 2 (606 AE 147 ng/L) than in Group 3 (2,192 AE 1,381 ng/L; P < 0.05). Serum folate concentrations (mean AE sd) were lower in Group 1 (9.6 AE 6.2 lg/L) and Group 2 (11.3 AE 6.3 lg/L) than in Group 3 (19.4 AE 7.9 lg/L; P < 0.05). Serum MMA concentrations (median [minimumÀmaximum]) were higher in Group 1 (239 [145À1,873] nmol/L) and Group 2 (559 [400À2,718] nmol/ L) than in Group 3 (147 [104À304] nmol/L; P < 0.05 and P < 0.01, respectively). Serum Hcy concentrations were not significantly different among the three groups (P = 0.13).