Selected article for: "expression cassette and TCR alpha"
Author: Banu, Nasirah; Chia, Adeline; Ho, Zi Zong; Garcia, Alfonso Tan; Paravasivam, Komathi; Grotenbreg, Gijsbert M.; Bertoletti, Antonio; Gehring, Adam J.
Title: Building and Optimizing a Virus-specific T Cell Receptor Library for Targeted Immunotherapy in Viral Infections
  • Document date: 2014_2_25
    • ID: 44w6omdp_8
    Snippet: Orientation of the TCR gene cassette significantly impacts expression. There is evidence that the orientation of the TCR alpha and beta genes within the cassette can impact their expression 19 . Therefore, we synthesized codon optimized constructs linked with the P2A cleavage site in both orientations and tested TCR expression in primary human T cells ( Fig. 2A) . Where available, we monitored TCR expression with beta chain specific antibodies an.....
    Document: Orientation of the TCR gene cassette significantly impacts expression. There is evidence that the orientation of the TCR alpha and beta genes within the cassette can impact their expression 19 . Therefore, we synthesized codon optimized constructs linked with the P2A cleavage site in both orientations and tested TCR expression in primary human T cells ( Fig. 2A) . Where available, we monitored TCR expression with beta chain specific antibodies and HLApentamers/tetramers. Interestingly, in 9/10 of the TCRs tested, expression of the introduced TCR was better (higher frequency of TCR1 cells) when the TCR beta gene was upstream of the TCR alpha gene in the expression cassette. This was true for staining the beta chain ( Fig. 2B&C ) or with HLA-pentamers/tetramers (Fig. 2E&F ).

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