Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system Document date: 2019_3_18
ID: 6nq7y1qe_2
Snippet: To overcome these obstacles we introduce the C3P3-G1 expression system, which relies on two components: (i) an artificial chimeric enzyme having both 5 -capping and RNA synthesis activities and (ii) DNA templates that are specifically transcribed by the transduced enzyme and provide artificial polyadenylation. Once C3P3-G1 enzyme is expressed in the cytoplasm, capped and polyadenylated mRNAs are produced independently of the host cell transcripti.....
Document: To overcome these obstacles we introduce the C3P3-G1 expression system, which relies on two components: (i) an artificial chimeric enzyme having both 5 -capping and RNA synthesis activities and (ii) DNA templates that are specifically transcribed by the transduced enzyme and provide artificial polyadenylation. Once C3P3-G1 enzyme is expressed in the cytoplasm, capped and polyadenylated mRNAs are produced independently of the host cell transcription machinery. We present the first generation of the C3P3-G1 system (C3P3-G1), which was optimized for mammalian cells. This system shows promising results for transient expression in some mammalian cell lines including CHO-K1, while less efficient in other cell lines and therefore requiring further optimization.
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