Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system Document date: 2019_3_18
ID: 6nq7y1qe_36
Snippet: The concentrations of released hEPO, hG-CSF and mAFP were measured in culture medium collected every single day. Protein concentrations were determined by enzyme-linked immunosorbent assay (ELISA) using commercial kits (Quantikine ELISA, R&D Systems, Minneapolis, MN, USA) according to the manufacturer's instructions. Results were expressed as the optical density at 450 nm, with wavelength correction set to 620 nm. Results were analyzed by 4-param.....
Document: The concentrations of released hEPO, hG-CSF and mAFP were measured in culture medium collected every single day. Protein concentrations were determined by enzyme-linked immunosorbent assay (ELISA) using commercial kits (Quantikine ELISA, R&D Systems, Minneapolis, MN, USA) according to the manufacturer's instructions. Results were expressed as the optical density at 450 nm, with wavelength correction set to 620 nm. Results were analyzed by 4-parameter nonlinear logistic regression algorithm (17) .
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