Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system Document date: 2019_3_18
ID: 6nq7y1qe_70
Snippet: We have optimized components of the DNA templates with no or modest effects of pause site, 5 -UTR, 3 -UTR, and 3 -stop sequences on luciferase expression (Supplementary Figures S14-17, Supplementary Table S7-8) . Conversely, the combination of poly[A]40 track and consensus histone stem-loop sequence, which is functionally similar to a poly(A) tail in that it enhances translational efficiency and is dependent on mRNA capping, markedly improved luc.....
Document: We have optimized components of the DNA templates with no or modest effects of pause site, 5 -UTR, 3 -UTR, and 3 -stop sequences on luciferase expression (Supplementary Figures S14-17, Supplementary Table S7-8) . Conversely, the combination of poly[A]40 track and consensus histone stem-loop sequence, which is functionally similar to a poly(A) tail in that it enhances translational efficiency and is dependent on mRNA capping, markedly improved luciferase expression ( Figure 3E ).
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