Selected article for: "host cell and observed change"
Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system
  • Document date: 2019_3_18
    • ID: 6nq7y1qe_72
    Snippet: To investigate if the above results could be caused by global suppression of host-cell translation, the polysome profile of HEK-293 cells expressing the Firefly Luciferase under control of C3P3-G1 or standard CMVpromoter-based nuclear expression plasmid were compared. Polysome profiling separates translated mRNAs on a sucrose gradient according to the number of bound ribosomes. No frank change in ribosome distribution patterns was observed betwee.....
    Document: To investigate if the above results could be caused by global suppression of host-cell translation, the polysome profile of HEK-293 cells expressing the Firefly Luciferase under control of C3P3-G1 or standard CMVpromoter-based nuclear expression plasmid were compared. Polysome profiling separates translated mRNAs on a sucrose gradient according to the number of bound ribosomes. No frank change in ribosome distribution patterns was observed between these two conditions ( Figure 10) , which supports the notion that expression by C3P3-G1 has no major impact on global translation of human HEK-293 cells.

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