Selected article for: "capping enzyme and dna polymerase"
Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system
  • Document date: 2019_3_18
    • ID: 6nq7y1qe_77
    Snippet: We present the C3P3-G1 artificial expression system optimized for its uses in cultured mammalian cells. To the best of our knowledge, this is the first artificial expression system that successfully couples transcription and posttranscription, which are processes of exquisite complexity in eukaryotes. C3P3-G1 relies on a single-unit chimeric enzyme that consists of the fusion of an mRNA capping enzyme with a DNA-dependent RNA polymerase. The RNA .....
    Document: We present the C3P3-G1 artificial expression system optimized for its uses in cultured mammalian cells. To the best of our knowledge, this is the first artificial expression system that successfully couples transcription and posttranscription, which are processes of exquisite complexity in eukaryotes. C3P3-G1 relies on a single-unit chimeric enzyme that consists of the fusion of an mRNA capping enzyme with a DNA-dependent RNA polymerase. The RNA polymerase moiety of the C3P3 enzyme transcribes in cellulo specific DNA templates under control of the C3P3 promoter and synthesizes RNA chains, which are subsequently capped by the capping enzyme moiety. In contrast, the polyadenylation of the C3P3 transcripts is synthesized by transcription of a polyadenosine track from the DNA templates. Therefore, once primed, the C3P3 expression system autonomously synthesizes RNA chains in the cytoplasm of the host-cell and generates the key modifications requested for their translation.

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