Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system Document date: 2019_3_18
ID: 6nq7y1qe_8
Snippet: Plasmids used for comparison with the standard transient expression system consisted of the ORF under consideration subcloned in the commercial pCMVScript plasmid, e.g. pCMVScript-Luciferase. The resulting construction therefore contained the IE1 human CMV promoter/enhancer, Kozak consensus sequence followed by the ORF, and late SV40 polyadenylation signal......
Document: Plasmids used for comparison with the standard transient expression system consisted of the ORF under consideration subcloned in the commercial pCMVScript plasmid, e.g. pCMVScript-Luciferase. The resulting construction therefore contained the IE1 human CMV promoter/enhancer, Kozak consensus sequence followed by the ORF, and late SV40 polyadenylation signal.
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