C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system

Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya

A current imperfection of the C3P3 system consists in the incomplete translational efficiency of C3P3 transcripts, i.e. the mRNA:luminescence ratio that is lower using the C3P3-G1 system than the standard nuclear expression system. Such incomplete mRNA translational efficiency might be explained by various mechanisms. Firstly, the mRNA The activity of hEPO produced by the C3P3-G1 system was comparable to the nuclear expression plasmid, therefore showing normal protein folding and disulfide found formation. The differences of activity profiles between Epoetin ␤ and hEPO produced in CHO-K1 cells with the standard plasmid or the C3P3-G1 system might be explained by the use of distinct methods for measuring hEPO or other differences in line with the processing of Epoetin ␤ product (20) .