Author: Xia, Shuai; Yan, Lei; Xu, Wei; Agrawal, Anurodh Shankar; Algaissi, Abdullah; Tseng, Chien-Te K.; Wang, Qian; Du, Lanying; Tan, Wenjie; Wilson, Ian A.; Jiang, Shibo; Yang, Bei; Lu, Lu
Title: A pan-coronavirus fusion inhibitor targeting the HR1 domain of human coronavirus spike Document date: 2019_4_10
ID: 3c5ab73l_66
Snippet: Cytotoxicity of the peptides to the cells (293 T, 293 T/ACE2, Huh-7, A549, LLC-MK2, and Calu-3 cells) was tested by using the Cell Counting Kit-8 (CCK-8; Dojindo, Kumamoto, Japan). Briefly, each cell type was seeded into the wells of a 96-well microtiter plate (10 4 per well) and incubated at 37°C for 12 hours, replacing medium with DMED containing EK1 at graded concentrations. After incubation at 37°C for 2 days, CCK-8 solution (10 ïl per we.....
Document: Cytotoxicity of the peptides to the cells (293 T, 293 T/ACE2, Huh-7, A549, LLC-MK2, and Calu-3 cells) was tested by using the Cell Counting Kit-8 (CCK-8; Dojindo, Kumamoto, Japan). Briefly, each cell type was seeded into the wells of a 96-well microtiter plate (10 4 per well) and incubated at 37°C for 12 hours, replacing medium with DMED containing EK1 at graded concentrations. After incubation at 37°C for 2 days, CCK-8 solution (10 ïl per well) was added, followed by an additional incubation for 4 hours. The absorbance was measured at 450 nm.
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