2015 ACVIM Forum Research Abstract Program

The neuroendocrine system of the gut plays a significant role in regulating intestinal motor and sensory functions, as well as modulating inflammation in the intestine. Serotonin (5-HT) acts locally on the gastrointestinal tract, and excess 5-HT has been shown in human beings to have a role in the pathophysiology of several gastrointestinal diseases. 5-Hydroxyindoleacetic acid (5-HIAA), the main stable metabolite of 5-HT, is excreted in the urine. Measurement of this compound can be used to determine degree of serotoninergic system activity in an individual, making it a potential marker of serotoninergic system activation in gastrointestinal disease. The aim of this study was to validate a commercially available 5-HIAA ELISA assay for use with canine urine, establish a reference range for 5-HIAA:Creatinine in urine samples from 20 clinically healthy dogs, and compare those values to those of dogs with enteropathic disease.