Author: Liu, Hong Yan; Gao, Xiaohu
Title: A Universal Protein Tag for Delivery of SiRNA-Aptamer Chimeras Document date: 2013_11_7
ID: 0atfsivf_14
Snippet: Cytotoxicity. Lastly, we probed the cytotoxicity of the best performing protein tag dsRBD-His 18 using a standard cell viability assay (CellTiter-BlueH). The assay is based on the ability of living cells to convert a redox dye (resazurin) into a fluorescent end product (resorufin). Nonviable cells lose metabolic capacity and thus do not generate fluorescent signals. As illustrated in Figure 5 , virtually no toxicity was detected up to a concentra.....
Document: Cytotoxicity. Lastly, we probed the cytotoxicity of the best performing protein tag dsRBD-His 18 using a standard cell viability assay (CellTiter-BlueH). The assay is based on the ability of living cells to convert a redox dye (resazurin) into a fluorescent end product (resorufin). Nonviable cells lose metabolic capacity and thus do not generate fluorescent signals. As illustrated in Figure 5 , virtually no toxicity was detected up to a concentration four times as high as the one used in the delivery work in reference to the untreated control. This is perhaps not too surprising due to the biocompatibility of dsRBD, a small protein of human origin. More importantly, for future in vivo applications, we envision that the small protein tag would have improved clearance capability compared with synthetic polymers and inorganic nanoparticles used for siRNA delivery.
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