Author: Liu, Hong Yan; Gao, Xiaohu
Title: A Universal Protein Tag for Delivery of SiRNA-Aptamer Chimeras Document date: 2013_11_7
ID: 0atfsivf_27
Snippet: Microscopy and flow cytometry studies of gene knockdown efficacy. C4-2 prostate cancer cells expressing GFP were seeded into 35 mM glass-bottom petri dishes for confocal imaging or 6-well plates for flow cytometry. Cells were treated with chimera & dsRBD-His 18 and compared with five control groups including no treatment, treated with GFP-siRNA alone, chimera alone, a random sequenced siRNA with the protein tag (His 18 ), and chimera with protein.....
Document: Microscopy and flow cytometry studies of gene knockdown efficacy. C4-2 prostate cancer cells expressing GFP were seeded into 35 mM glass-bottom petri dishes for confocal imaging or 6-well plates for flow cytometry. Cells were treated with chimera & dsRBD-His 18 and compared with five control groups including no treatment, treated with GFP-siRNA alone, chimera alone, a random sequenced siRNA with the protein tag (His 18 ), and chimera with protein tag (His 6 ) for 2 h in serum free media and then incubated in complete media for 60 h. Confocal images were again obtained with LSM 510 confocal microscope equipped with argon (488 nm) and HeNe (543 nm) lasers; and quantitative flow cytometry investigation was done on a BD FACSCantoII flow cytometer.
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