Author: Ivanova, Elena; Berger, Audrey; Scherrer, Anne; Alkalaeva, Elena; Strub, Katharina
Title: Alu RNA regulates the cellular pool of active ribosomes by targeted delivery of SRP9/14 to 40S subunits Document date: 2015_3_11
ID: 64cnoqpi_26
Snippet: We next studied the effect of Alu RNPs on the assembly of different translation complexes from purified mammalian translation factors and high-salt purified ribosomal subunits (18) . The assembly efficiency was determined by primer extension inhibition (toeprinting) using fluorescently labeled DNA primers and capillary electrophoresis ( Figure 1D ) (19, 20) ......
Document: We next studied the effect of Alu RNPs on the assembly of different translation complexes from purified mammalian translation factors and high-salt purified ribosomal subunits (18) . The assembly efficiency was determined by primer extension inhibition (toeprinting) using fluorescently labeled DNA primers and capillary electrophoresis ( Figure 1D ) (19, 20) .
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