Selected article for: "blot analysis and RNA bind"
Author: Ivanova, Elena; Berger, Audrey; Scherrer, Anne; Alkalaeva, Elena; Strub, Katharina
Title: Alu RNA regulates the cellular pool of active ribosomes by targeted delivery of SRP9/14 to 40S subunits
  • Document date: 2015_3_11
    • ID: 64cnoqpi_58
    Snippet: We next wanted to determine whether in the cell an increase in Alu RNP levels could influence translation efficiency. We chose to express the short scAlu RNA in HEK 293T cells, because it is more stable than Alu RNA (26) and because it lacks the inhibitory activities in Pol II-dependent transcription and in PKR activation (9, 27, 28) . The overexpressed scAluY NF1 RNA (Table 1) is expected to bind SRP9/14, since human cells have a large pool of f.....
    Document: We next wanted to determine whether in the cell an increase in Alu RNP levels could influence translation efficiency. We chose to express the short scAlu RNA in HEK 293T cells, because it is more stable than Alu RNA (26) and because it lacks the inhibitory activities in Pol II-dependent transcription and in PKR activation (9, 27, 28) . The overexpressed scAluY NF1 RNA (Table 1) is expected to bind SRP9/14, since human cells have a large pool of free SRP9/14 (11) . As controls, we expressed 7SL RNA and 4.5S RNA, a 90 nucleotide-long, rodent-specific RNA, which fails to bind SRP9/14 (29) . Expression of the RNAs was confirmed by northern blot analysis ( Figure 6B ) which showed that levels of scAluY NF1 RNA and 7SL RNA were increased 5-10 and 1.5-2-fold respectively, compared to the corresponding endogenous RNA levels.

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