2015 ACVIM Forum Research Abstract Program

MARKER: URINARY CLUSTERIN (UCLUS). Jack Quinn, Mirolee Zieba, Murthy Yerramilli. IDEXX Laboratories Inc, Westbrook, ME, USA Clusterin or Apolipoprotein J is a 75-80 kDa disulphide linked heterodimeric protein. Clusterin has been shown to be upregulated during proximal and distal tubular injury. uClus is part of the FDA and ICH renal toxicity biomarker panels for drug development. Clusterin changes are more profound and significant in urine compared to serum during kidney injury events. A commercial EIA kit from Biovendor is available for the quantification of canine clusterin in both serum and urine. A recent study validated the biomarker using this kit in dogs with leishmaniasis. In healthy dogs serum concentrations of clusterin are 1000-fold higher (60-70 lg/mL) than the urinary concentrations (<100 ng/mL). It has been reported that uClus is an AKI biomarker which increases during and, as a result of, kidney injury in dogs.