Title: 2015 ACVIM Forum Research Abstract Program Document date: 2015_5_27
ID: 3pnuj5ru_873
Snippet: Sixteen healthy horses with no recent history of underlying disease were used for this study. They were randomly assigned to one of four treatment groups (n = 4 horses per group). Each group was treated intravenously with antibiotics (ceftiofur, enrofloxacin, oxytetracycline) or saline (control) at standard doses for five consecutive days. Fecal samples were collected once daily during treatment. Bacterial DNA was extracted, PCR amplified and ana.....
Document: Sixteen healthy horses with no recent history of underlying disease were used for this study. They were randomly assigned to one of four treatment groups (n = 4 horses per group). Each group was treated intravenously with antibiotics (ceftiofur, enrofloxacin, oxytetracycline) or saline (control) at standard doses for five consecutive days. Fecal samples were collected once daily during treatment. Bacterial DNA was extracted, PCR amplified and analyzed by 454 pyrosequencing of the 16S rRNA gene from baseline (before treatment) three, five and 30 days post-treatment. The composition and diversity of the microbiota were determined and compared using MOTHUR. Linear mixed models were used to analyze differences between treatment groups and time points using subject as a random effect.
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