2016 ACVIM Forum Research Abstract Program

Bcl11b staining intensity was moderate to strong in 79.4% (27/ 34) of FOSCC's. FOSCC biopsies with intense Bcl11b staining had the highest number of Bcl11b positive cells (P < 0.001). All biopsies expressed VEGF-D and 85.7% (18/21) expressed EGFR at varying intensities. A correlation between increasing Bcl11b and EGFR expression approached significance (P = 0.05598). All FOSCC cell lines expressed pBcl11b, pEGFR, pSrc, pERK, and VEGF-D. Dasatinib treatment lowered cell viability, inhibited activation of Bcl11b, and suppressed VEGF production. Ligand stimulation of EGFR resulted in increased activation of EGFR and ERK, increased VEGF/VEGF-D production, and partially rescued FOSCC cells from the effects of dasatinib.