Title: 2015 ACVIM Forum Research Abstract Program Document date: 2015_5_27
ID: 3pnuj5ru_236
Snippet: Western blot and immunohistochemistry identified NQO1 in cell lines and an enzymatic activity assay determined the presence of a functional protein. One cell line per tumor histology was selected to demonstrate a temporal and dose-dependent regulation of NQO1 by radiation. The cytotoxic effects of irradiation and DNQ were investigated by treating selected cell lines and measuring surviving fraction of cells in conjunction with fluorescent microsc.....
Document: Western blot and immunohistochemistry identified NQO1 in cell lines and an enzymatic activity assay determined the presence of a functional protein. One cell line per tumor histology was selected to demonstrate a temporal and dose-dependent regulation of NQO1 by radiation. The cytotoxic effects of irradiation and DNQ were investigated by treating selected cell lines and measuring surviving fraction of cells in conjunction with fluorescent microscopy to identify double-stranded DNA breaks via the biomarker gammaH2AX.
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