2015 ACVIM Forum Research Abstract Program

The two cats orally inoculated with B. henselae infected C. felis and frass had no evidence of dental disease or stomatitis and did not develop evidence of infection over the time period studied with the defined assays. These results suggest that normal cats ingesting B. henselae infected C. felis are unlikely to become infected. However, further experiments should be performed to assess this route of transmission using more sensitive assays like BAPGM culture plus PCR assay and to use kittens that are teething or cats affected by oral cavity disease to provide a potential portal of entry for B. henselae. Bacterial cystitis is common in dogs as both a primary disorder and as a complication of co-morbid conditions. An increase in urinary isolates resistant to antimicrobial drugs has been reported in recent years. Multiple drug resistant (MDR) organisms, defined as organisms with acquired resistance to at least one member of 3 or more classes of antimicrobials, seem to be commonplace in the Veterinary Medical Diagnostic Laboratory at the University of Missouri. We undertook a descriptive retrospective study of drug resistance of urinary isolates from the MU-VMDL from Jan. 1, 2014 1, to Nov. 19, 2014 Urine cultures were performed using calibrated loops delivering urine to both blood agar and MacConkey agar plates for aerobic bacterial isolation. Plates were incubated under routine culture conditions. Antimicrobial susceptibility testing of resultant isolates was accomplished with the MIC technique using commercially prepared lyophilized trays. Breakpoints for determination of resistance or susceptibility were based on the standards set by the National Committee for Clinical Laboratory Standards. Antimicrobials included on routine susceptibility profiles differed slightly depending on organism (to exclude intrinsic resistance) but included aminoglycosides (amikacin and gentamicin), potentiated penicillin and carboxypenicillin (amoxicillin-clavulanate, ticarcillin-clavulanate), natural and synthetic penicillins and carboxypenicillin (ampicillin, penicillin, oxacillin +2% NACL, ticarcillin), 1st generation cephalosporins (cefazolin, cephalothin), 2nd generation cephalosporins (cefoxitin, ceftiofur), 3rd generation cephalosporins (cefovecin, cefpodoxime), chloramphenicol, clindamycin, doxycycline, erythromycin, fluoroquinolones (enrofloxacin, marbofloxacin), imipenem, rifampin, and trimethoprim sulfa (TMS). The 4 most common isolates (E. coli, Staphylococcus spp., Enterococcus spp., and Streptococcus spp) were evaluated. Innate antimicrobial resistance was defined for each of the four microbes of interest as follows: E. coliclindamycin, erythromycin, penicillin; Staphylococcus spp.aminoglycosides; Streptococcus spp.aminoglycosides; Enterococcus spp.aminoglycosides, all generations of cephalosporins, and TMS.