Selected article for: "conventional PCR assay and PCR assay"

Title: 2015 ACVIM Forum Research Abstract Program
  • Document date: 2015_5_27
  • ID: 3pnuj5ru_543
    Snippet: Dogs (n = 72) being sterilized at a low cost clinic in Checotah, OK during July 2014 were selected as those likely to be exposed to Ambloymma americanum and so at high risk for E. ewingii and E. chaffeensis exposure. Rhipicephalus sanguineus also is found in the region and so E. canis infection was also possible. A total of 1 mL of blood in EDTA collection tubes was stored in a refrigerator overnight, and the next day were sent on cold packs to t.....
    Document: Dogs (n = 72) being sterilized at a low cost clinic in Checotah, OK during July 2014 were selected as those likely to be exposed to Ambloymma americanum and so at high risk for E. ewingii and E. chaffeensis exposure. Rhipicephalus sanguineus also is found in the region and so E. canis infection was also possible. A total of 1 mL of blood in EDTA collection tubes was stored in a refrigerator overnight, and the next day were sent on cold packs to the first testing laboratory via overnight shipping. On arrival, the samples were split into 2 aliquots with 1 aliquot being frozen at -80C and 1 aliquot being prepared for evaluation in a commercially available conventional Ehrlichia spp. PCR assay (www.dlab.colostate.edu). The frozen EDTA blood was shipped to the second PCR laboratory on dry ice for evaluation in a commercially available real time multiplex Ehrlichia spp. PCR assay (www.antech.com). Results from samples with sequence confirmation were compared using the kappa statistic.

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