2016 ACVIM Forum Research Abstract Program

In summary, the majority of dogs that develop PMM do so within 3 days of presentation and progress to euthanasia within another 3 days. However, onset can be delayed up to 5 days after presentation with progression to death taking as long as 2 weeks. Non-specific systemic signs are commonly reported. Benzimidazole anthelmintic drugs have been reported to have antiproliferative effects on several cancers both in vitro and in vivo, with reduced off-target toxicity as compared to other microtubuledisrupting drugs. In addition, these small, lipophilic drugs readily cross the blood-brain barrier. The purpose of this study was to evaluate the in vitro chemosensitivity of canine glioblastoma J3T cells to mebendazole (MBZ) and fenbendazole (FBZ). Cells were exposed to drugs for 72 hours, and cell viability was evaluated using the MTT assay. The half-maximal inhibitory concentration (IC 50 ) of MBZ and FBZ was calculated using a fourparameter variable slope curve fit nonlinear regression analysis. The IC 50 was compared between the two drugs. Western blot was used to compare the ratio of polymerized to depolymerized tubulin between drug-treated cells and untreated controls. Immunocytochemistry was performed on treated and untreated cells to visualize effects of treatment on tubulin.