Title: 2016 ACVIM Forum Research Abstract Program Document date: 2016_5_31
ID: 2y1y8jpx_392
Snippet: Serum and EDTA-anticoagulated blood samples were collected from healthy control dogs (HC), dogs with primary IMHA (IMHA), and dogs with inflammatory diseases (INF). Dogs were considered to have primary IMHA if they were anemic (with hematocrit less than 35%) and fulfilled one of the following criteria: significant spherocytosis on fresh blood smear, persistent agglutination of erythrocytes after dilution in saline, or a titer of greater than 1:16.....
Document: Serum and EDTA-anticoagulated blood samples were collected from healthy control dogs (HC), dogs with primary IMHA (IMHA), and dogs with inflammatory diseases (INF). Dogs were considered to have primary IMHA if they were anemic (with hematocrit less than 35%) and fulfilled one of the following criteria: significant spherocytosis on fresh blood smear, persistent agglutination of erythrocytes after dilution in saline, or a titer of greater than 1:16 in a direct antiglobulin test. Dogs were excluded if any underlying cause of disease was detected by serum biochemical testing, imaging of the thorax and abdomen and testing for infectious agents. Dogs in the INF group had diseases that were not considered to have an immune-mediated etiology and had no previous history of autoimmune disease. Dogs in the HC group had no previous history of autoimmune disease and had unremarkable physical examinations. Owners of dogs consented for collection and use of samples, and the study was approved by an institutional ethical review committee.
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