2018 ACVIM Forum Research Abstract Program: Seattle, Washington, June 14 - 15, 2018

Twenty diarrheic calves (DC) (ages of 1 to 30 days) and 20 agematched healthy control calves (HC) were enrolled. Viral nucleic extraction was performed using a commercial Kit. Synthesis of viral cDNA was completed using a primer containing a fixed 18bp plus a random nonamer. Twenty samples from HC were mixed in equimolar quantities in 4 sets as well as 20 DC samples. The 8 total pooled samples were sequenced using MiSeq-lllumina-Platform. Raw sequences quality was assessed using FastQC. Then, low-quality reads were removed with Trimmomatic. CD-HIT-EST was used to cluster reads with >90% sequence identity. The five more representative sequences of each cluster were used to perform a de novo assembly using SPADES. LefSe was used to obtain molecular markers, which can be used in diagnosis.