2018 ACVIM Forum Research Abstract Program: Seattle, Washington, June 14 - 15, 2018

Intra-assay (6 samples) and inter-assay (7 samples) coefficients of variation (%CVs) ranged from 1.4% to 2.7%, and 3.4% to 5.8%, respectively. Observed to expected ratios for mixtures of two serum samples of known concentrations (15 pairs), ranged from 91.5% to 128.6% with a mean ± SD of 101.3% ± 8.3%. Comparison with the Tridelta assay showed a non-linear relationship (R-squared = 0.46). This was especially apparent for values greater than 30 mg/L (reference interval < 7.6 mg/L) when measured by the Tridelta assay, where the Gentian results were higher than expected). Using the suggested cut-off values of 10.0 mg/L for the Gentian assay and 7.6 mg/L for the Tridelta assay, agreement between results was moderate (Cohen'; s kappa statistic = 0.52).