Title: 2018 ACVIM Forum Research Abstract Program: Seattle, Washington, June 14 - 15, 2018 Document date: 2018_10_25
ID: 60ceejq1_461
Snippet: The direct flow cytometry assay was first optimized and a cut-off value of ≤ 10% to differentiate negative and positive classifications was determined by serial direct flow evaluation of platelets from a healthy dog. The assay was then performed on samples from 9 healthy beagles and 12 client-owned thrombocytopenic dogs at four time points: fresh and 24, 48, and 72 hours after storage at 4 C and the % IgG was recorded. A repeated measures analy.....
Document: The direct flow cytometry assay was first optimized and a cut-off value of ≤ 10% to differentiate negative and positive classifications was determined by serial direct flow evaluation of platelets from a healthy dog. The assay was then performed on samples from 9 healthy beagles and 12 client-owned thrombocytopenic dogs at four time points: fresh and 24, 48, and 72 hours after storage at 4 C and the % IgG was recorded. A repeated measures analysis (one-way ANOVA) was performed and storage time points were compared to fresh samples using Dunnett';s method. The average coefficient of variation (CV) was calculated for samples from thrombocytopenic dogs to evaluate intra-assay variability.
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