Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system Document date: 2019_3_18
ID: 6nq7y1qe_11
Snippet: Cells were routinely plated in 24-well plates at 1 × 10 5 cells per well the day before transfection and transfected at 80% cell confluence. Transient transfection was performed with Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA) according to manufacturer's recommendations, except when otherwise stated. For standard luciferase and hSEAP gene reporter expression assays, cells were analyzed 24 h after transfection......
Document: Cells were routinely plated in 24-well plates at 1 × 10 5 cells per well the day before transfection and transfected at 80% cell confluence. Transient transfection was performed with Lipofectamine 2000 reagent (Invitrogen, Carlsbad, CA, USA) according to manufacturer's recommendations, except when otherwise stated. For standard luciferase and hSEAP gene reporter expression assays, cells were analyzed 24 h after transfection.
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