Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system Document date: 2019_3_18
ID: 6nq7y1qe_29
Snippet: Cell viability was assayed according to the manufacturer's instructions after addition of 50 l of the live-cell dipeptidyl peptidase-1 GF-AFC reagent and analyzed on a fluorescent plate reader using wavelengths of 400 and 505 nm for excitation and emission, respectively. The GF-AFC reagent enters intact cells, where it is cleaved by the dipeptidyl peptidase-1, an enzyme that is restricted to intact viable cells, which release AFC and generate a f.....
Document: Cell viability was assayed according to the manufacturer's instructions after addition of 50 l of the live-cell dipeptidyl peptidase-1 GF-AFC reagent and analyzed on a fluorescent plate reader using wavelengths of 400 and 505 nm for excitation and emission, respectively. The GF-AFC reagent enters intact cells, where it is cleaved by the dipeptidyl peptidase-1, an enzyme that is restricted to intact viable cells, which release AFC and generate a fluorescent signal that is proportional to the number of viable cells (10) . This live-cell protease becomes inactive upon loss of membrane integrity and leakage into the surrounding culture medium.
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