Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system Document date: 2019_3_18
ID: 6nq7y1qe_35
Snippet: The production yields of secreted or cytoplasmic/secreted proteins were assayed in cell culture medium of CHO-K1 cells. To avoid cross-reactivity in ELISA assay measurements, CHO-K1 cells were cultured and transfected in Panserin PX10 serum-free medium (Pan-Biotech, Aidenbach, Germany), supplemented with 1% penicillin and streptomycin, and 0.25% fungizone. Wild-type ORFs encoding protein precursors were artificially synthesized, i.e. human erythr.....
Document: The production yields of secreted or cytoplasmic/secreted proteins were assayed in cell culture medium of CHO-K1 cells. To avoid cross-reactivity in ELISA assay measurements, CHO-K1 cells were cultured and transfected in Panserin PX10 serum-free medium (Pan-Biotech, Aidenbach, Germany), supplemented with 1% penicillin and streptomycin, and 0.25% fungizone. Wild-type ORFs encoding protein precursors were artificially synthesized, i.e. human erythropoietin, human granulocyte colonystimulating factor and murine alpha-fetoprotein, and subcloned in cassette optimized for the C3P3-G1 enzyme or standard pCMVScript nuclear expression plasmids (Supplementary Table S2 ).
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