Selected article for: "absolute number and macherey nagel"
Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system
  • Document date: 2019_3_18
    • ID: 6nq7y1qe_43
    Snippet: Total RNA was isolated using the Nucleospin RNA columns (Macherey-Nagel, Düren, Germany) and subjected to TURBO DNase-free treatment (Ambion, Foster City, CA, USA). Total RNA was reverse-transcribed using High Capacity cDNA Reverse Transcription kit with RNase Inhibitor (Life Technologies). The resulting cDNA was amplified by real-time RT-PCR using primer and probe sets for either Luciferase ORF or C3P3-G1 enzyme (Supplementary Table S3 ) with T.....
    Document: Total RNA was isolated using the Nucleospin RNA columns (Macherey-Nagel, Düren, Germany) and subjected to TURBO DNase-free treatment (Ambion, Foster City, CA, USA). Total RNA was reverse-transcribed using High Capacity cDNA Reverse Transcription kit with RNase Inhibitor (Life Technologies). The resulting cDNA was amplified by real-time RT-PCR using primer and probe sets for either Luciferase ORF or C3P3-G1 enzyme (Supplementary Table S3 ) with TaqMan detection. Absolute transcript copy number was reported to calibration curve with known concentrations of each plasmid DNA and normalized to human GAPDH or ACTB expression.

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