Selected article for: "calibration curve and copy number"

Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system
  • Document date: 2019_3_18
  • ID: 6nq7y1qe_43
    Snippet: Total RNA was isolated using the Nucleospin RNA columns (Macherey-Nagel, Düren, Germany) and subjected to TURBO DNase-free treatment (Ambion, Foster City, CA, USA). Total RNA was reverse-transcribed using High Capacity cDNA Reverse Transcription kit with RNase Inhibitor (Life Technologies). The resulting cDNA was amplified by real-time RT-PCR using primer and probe sets for either Luciferase ORF or C3P3-G1 enzyme (Supplementary Table S3 ) with T.....
    Document: Total RNA was isolated using the Nucleospin RNA columns (Macherey-Nagel, Düren, Germany) and subjected to TURBO DNase-free treatment (Ambion, Foster City, CA, USA). Total RNA was reverse-transcribed using High Capacity cDNA Reverse Transcription kit with RNase Inhibitor (Life Technologies). The resulting cDNA was amplified by real-time RT-PCR using primer and probe sets for either Luciferase ORF or C3P3-G1 enzyme (Supplementary Table S3 ) with TaqMan detection. Absolute transcript copy number was reported to calibration curve with known concentrations of each plasmid DNA and normalized to human GAPDH or ACTB expression.

    Search related documents:
    Co phrase search for related documents
    • calibration curve and known concentration: 1, 2
    • calibration curve and real time: 1, 2, 3, 4, 5, 6
    • cdna Reverse Transcription kit and High Capacity cdna Reverse Transcription kit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14
    • cdna Reverse Transcription kit and plasmid dna: 1
    • cdna Reverse Transcription kit and probe primer: 1, 2
    • cdna Reverse Transcription kit and probe primer set: 1
    • cdna Reverse Transcription kit and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10
    • cdna Reverse Transcription kit and result cdna: 1
    • cdna Reverse Transcription kit and Transcription kit: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24
    • copy number and known concentration: 1
    • copy number and plasmid dna: 1, 2, 3, 4, 5, 6, 7
    • copy number and probe primer: 1, 2, 3, 4, 5
    • copy number and probe primer set: 1
    • copy number and real time: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24, 25, 26, 27, 28, 29, 30, 31, 32, 33, 34, 35, 36, 37, 38, 39, 40, 41, 42, 43, 44, 45, 46, 47, 48, 49, 50, 51, 52, 53
    • copy number and result cdna: 1
    • copy number and TaqMan detection: 1
    • copy number and transcript copy number: 1, 2, 3, 4
    • copy number and Transcription kit: 1, 2
    • High Capacity cdna Reverse Transcription kit and plasmid dna: 1