Author: Jaïs, Philippe H; Decroly, Etienne; Jacquet, Eric; Le Boulch, Marine; Jaïs, Aurélien; Jean-Jean, Olivier; Eaton, Heather; Ponien, Prishila; Verdier, Fréderique; Canard, Bruno; Goncalves, Sergio; Chiron, Stéphane; Le Gall, Maude; Mayeux, Patrick; Shmulevitz, Maya
Title: C3P3-G1: first generation of a eukaryotic artificial cytoplasmic expression system Document date: 2019_3_18
ID: 6nq7y1qe_68
Snippet: Immunofluorescence microcopy showed only cytoplasmic expression of the FLAG-tagged NP868R-(G 4 S) 2 -K1ERNAP(R551S) enzyme as expected ( Figure 6A -B). In addition, the FLAG-tagged NP868R-(G 4 S) 2 -K1ERNAP(R551S) enzyme was expressed in both CHO-K1 and HEK-293 cells as the expected 200-kDa fusion protein ( Figure 6C ). Cell viability and death, measured by fluorescent dipeptidyl peptidase-1 GF-AFC and luminogenic AAF-aminoluciferin substrates re.....
Document: Immunofluorescence microcopy showed only cytoplasmic expression of the FLAG-tagged NP868R-(G 4 S) 2 -K1ERNAP(R551S) enzyme as expected ( Figure 6A -B). In addition, the FLAG-tagged NP868R-(G 4 S) 2 -K1ERNAP(R551S) enzyme was expressed in both CHO-K1 and HEK-293 cells as the expected 200-kDa fusion protein ( Figure 6C ). Cell viability and death, measured by fluorescent dipeptidyl peptidase-1 GF-AFC and luminogenic AAF-aminoluciferin substrates respectively, were similar for empty plasmids and C3P3-expressing plasmids, indicating that the C3P3-G1 enzyme do not exert any toxic effects on HEK-293 cells (Supplementary Figure S12) .
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